Manual

Product Number: TQE01-96T

Shipping and Storage

1. 2-8℃, stored away from light and moisture
2. Validity period: 6 months

Components

Note:20×Buffer WB:Dilute distilled water at a ratio of 1:20, which is 1 part of 20×Buffer WB and 19 parts of distilled water.

Description

The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the sample and HRP labeled detection antibody to the pre coated micro well with tetanus antigen, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and compare it with the CUTOFF value to determine the presence or absence of tetanus IgG antibody in the specimen.

Sample collection and storages

1. Serum: Use test tubes free of pyrogens and endotoxins, avoid any cell irritation during the operation, collect blood, centrifuge at 3000 rpm for 10 minutes, and quickly and carefully separate serum and red blood cells.
2. Plasma: EDTA、Citrate or heparin anticoagulant. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant.
3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers.
4. Tissue homogenate: Crush the tissue by adding an appropriate amount of physiological saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant.
5. Storage: If the sample is not tested in a timely manner after collection, please divide it into batches according to a single dose, freeze it at -20 ℃, avoid repeated freezing and thawing, thaw it at room temperature, and ensure that the sample is evenly filled and thawed.

Materials required but not supplied

1. ELISA reader (450nm)
2. High precision sampler and nozzle: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL
3. 37 ℃ constant temperature box

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