Bacterial genomic Medium Kit
2024-12-02Plant DNA Mini Kit
2024-12-05Product Number: DNK1401
Shipping and Storage
- Buffer PL or Buffer IR may precipitate and precipitate at low temperatures. It can be dissolved again by taking a water bath at 55℃ for a few minutes, restoring clarity and transparency, and then cooled to room temperature before use.
- Avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air. Each solution should be covered tightly after use.
Components
| Component | Storage | DNK1401 50 Preps | DNK1402 100 Preps | DNK1403 200 Preps |
| RNase A | 4°C | 250 μl | 500 μl | 1ml |
| Buffer PL | RT | 30 ml | 60 ml | 120 ml |
| Buffer PQ | RT | 18 ml | 35 ml | 70 ml |
| Add the specified amount of ethanol according to the bottle label instructions before the first use. | ||||
| Buffer PE | RT | 25 ml | 50 ml | 100 ml |
| Add the specified amount of ethanol according to the bottle label instructions before the first use. | ||||
| Buffer WB | RT | 13 ml | 25 ml | 50 ml |
| Add the specified amount of ethanol according to the bottle label instructions before the first use. | ||||
| Buffer EB | RT | 15 ml | 15 ml | 20 ml |
| Adsorption column AC | RT | 50 | 100 | 200 |
| Collection tube (2ml) | RT | 50 | 100 | 200 |
Note: Storing this reagent kit at room temperature for 12 months does not affect its effectiveness.
Description
The improved classic CTAB plant DNA extraction solution (with the addition of various polysaccharide and polyphenol removal components tailored to plant characteristics) rapidly lyses cells and inactivates intracellular nucleases. After chloroform extraction, polysaccharides, polyphenols, and proteins are removed by centrifugation (as needed, isopropanol is also added to the supernatant to precipitate genomic DNA and further remove other impurities), then, the genomic DNA is selectively adsorbed onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, impurities such as polysaccharides, polyphenols, cellular metabolites, proteins, etc. are further removed. Finally, pure genomic DNA is eluted from the silica matrix membrane in a low salt elution buffer.
Features
- The silicon matrix membranes inside the centrifugal adsorption column are all made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
- Widely applicable, it can extract the vast majority of samples with high quality, including the most complex polysaccharide polyphenols and starch seed samples.
- Fast and simple, the operation of a single sample can generally be completed within 30 min.
- Several types of polysaccharides and polyphenols are removed, and multiple column washes are used to ensure high purity. The typical OD260/OD280 ratio is 1.7-1.9, with a length of up to 20kb-50kb. It can be directly used for PCR, Southern blot, and various enzyme digestion reactions.
Application
Suitable for rapid extraction of plant tissues (including complex polysaccharide and phenolic plants) and fungal genomic DNA.
