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2025-03-19Paenibacillus mucilaginosus Probe-Based qPCR Kit
Product Number: DTK555
Shipping and Storage
Low temperature transportation, stored at -20℃, with a shelf life of 24 months.
Component
| Component | Specification | Nine hole box packaging |
| 2×Probe qPCR MagicMix | 0.5mL | Natural color tube |
| Fluorescent PCR specific template diluent | 1mL | Green cover |
| Ultrapure water | 1mL | Blue cover |
| Gel like Bacillus subtilis qPCR Primer Probe Dry Powder | 50 | Brown cover |
| qPCR of gelatinous Bacillus subtilis Positive control (1E7 copy/μL) | 50μL | Yellow cover |
Note: Primer probe dry powder needs to be briefly centrifuged before use, and then 165μL of ultrapure water should be added to the centrifuge tube and thoroughly mixed before use. Unused samples need to be stored at -20℃.
Description
Paenibacillus mucilaginosus is a beneficial microorganism and an important functional bacterium in soil. It has the advantages of improving soil fertility, promoting plant growth, and improving soil structure. In agricultural production, gelatinous Bacillus subtilis is often used in the research and application of microbial fertilizers, which has a certain positive effect on improving crop yield and quality. Therefore, rapid detection of gelatinous Bacillus subtilis is of great significance.
Application
Our company has developed a detection kit for gel like Bacillus subtilis based on qPCR technology, which has the following characteristics:
- Ready to use, users only need to provide a sample DNA template.
- Primers and probes have been optimized for high analytical sensitivity, reaching up to 100 copies per reaction.
- Provide positive controls to distinguish false negative samples.
- High specificity, primers are designed based on highly conserved regions of Bacillus subtilis DNA, and will not cross react with DNA from other organisms.
- It can be used for both qualitative and quantitative testing. The linear range for quantitative detection should be at least 5 orders of magnitude.
- This product is sufficient for 50 qPCR reactions using a 20μL probe system.
- This product can only be used for scientific research.
Specimen collection
Sample DNA.
