Magbead Viral DNA/RNA Kit
2024-10-24
Fast NGS Library Kit for Illumina
2024-10-242×HotStar Probe Mixture (UNG)
Product Number: PCM63
Shipping and Storage
-20℃. If frequently used, it can be stored at 2-8℃ to avoid repeated freeze-thaw as much as possible.
Components
| Component | PCM63 5mL | PCM63L 5mL | PCM63H 5mL |
| 2×HotStar Probe Mixture (UNG) | 5×1mL | 5×1mL | 5×1mL |
| 50×Low ROX | - | 200μl | - |
| 50×High ROX | - | - | 200μl |
| ddH2O | 5×1mL | 5×1mL | 5×1mL |
Description
2× HotStar Probe Mixture (UNG) is a premixed system specifically designed for real-time fluorescence quantitative PCR using probe methods (TaqMan, Molecular Beacon, etc.), with a concentration of 2×,Contains Golden Star Taq DNA Polymerase, PCR Buffer, dNTPs (dTTP is completely replaced by dUTP), UNG enzyme, and Mg2+, making the operation simple and convenient.Mainly used for detecting genomic DNA target sequences and cDNA target sequences after RNA reverse transcription, such as gene expression analysis, copy number analysis, SNP genotype analysis, etc.This product uses the dUTP-UNG anti pollution system, and dUTP is added during the preparation process of the PCR reaction system, resulting in the formation of amplification products containing dU bases.And this product can be eliminated by UNG enzyme treatment in the PCR system before the next PCR reaction. This effectively removes residual contamination of PCR products and greatly reduces false positives caused by amplification product contamination.The pre denaturation step of UNG enzyme in the PCR cycle can be inactivated, so it will not affect the formation of new dU based PCR products.The Golden Star Taq DNA Polymerase contained in this product is a chemically modified, novel and highly efficient hot start enzyme that has no polymerase activity at room temperature, effectively avoiding non-specific amplification caused by non-specific binding of primers and templates or primer dimers at room temperature. The activation of the enzyme must be incubated at 95℃ for 10 minutes. The unique combination of PCR buffer system and hot start enzyme significantly improves the amplification efficiency of PCR, with stronger fluorescence signal and higher sensitivity, which can detect single copy templates. Using this product can obtain a wider linear range and more accurate quantification of the target gene.
ROX dye is used to correct the fluorescence signal errors generated from hole to hole in quantitative PCR instrument. It is generally used in ABI, Stratagene and other companies' Real Time PCR amplifiers. The excitation optical system varies from instrument to instrument, so the concentration of ROX dye must be matched with the corresponding fluorescence quantitative PCR instrument.
Instruments without ROX correction:Roche LightCycler 480, Roche LightCyler 96, Bio-rad iCyler iQ, iQ5, CFX96, etc.
Instruments requiring Low ROX correction:ABI Prism7500/7500 Fast, QuantStudio® 3 System, QuantStudio® 5 System, QuantStudio® 6 Flex System, QuantStudio® 7 Flex System, ViiA 7 system, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000, etc.
Instruments requiring High ROX calibration:ABI Prism7000/7300/770/7900, Eppendorf, ABI Step One/Step One Plus, etc.
Notes
- Before use, please gently mix it upside down and avoid foaming as much as possible. After briefly centrifuging, use it.
- Avoid repeated freeze-thaw of this product, as repeated freeze-thaw may cause a decrease in product performance. This product can be stored for a long time at -20℃, away from light. If frequent use is required in the short term, it can be stored at 2-8℃.
