The 2×SYBR qPCR Master Mix include the HotStar DNA Polymerase, PCR Buffer, MgCl2 , dNTPs, SYBR Green I, ROX and the stabilizing agent. The HotStar Taq DNA polymerase which in the mix is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is activated in 10 minute incubation at 95°C. experiments. The Rox in the mix can be used for the correction of the fluorescent signal in the qPCR. The 2×SYBR qPCR Master Mix produces a optimal results in qPCR.
For Protocal pls reference the attachment
Note: The functional activity of the enzyme should to be activated in 10 minute incubation at 95°C