The 2×TaqMan Master Mix is a special mixture for Probe real time PCR(TaqMan, Molecular Beacon etc.). Which include the HotStar DNA Polymerase, PCR Buffer, MgCl2 , dNTPs, ROX and the stabilizing agent. The HotStar Taq DNA polymerase which in the mix is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is activated in 10 minute incubation at 95°C. The unique reaction buffer with the special Hotstar enzyme highly increased the Amplification efficiency. The fluorescence signals is stronger, the sensitivity is higher can detect the Single copy sequences. The Rox in the mix can be used for the correction of the fluorescent signal in the qPCR. The 2×TaqMad qPCR master Mix produces a optimal results in qPCR.
Store at -20℃