This Taq PCR Master mix contains Taq DNA Polymerase, dNTPs, and optimized buffer, and blue dye. This Taq Mastermix is provided at 2× concentration and used at 1× concentration by adding the template, primer, and H2O. It has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1-2 kb/min. There is an “A” on the 3′end. The PCR product can be cloned in a TA vector.
This product has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >90% homogeneous by SDS gel electrophoresis. Each lot of Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA.
Store at -20°C
no repeated freezing and thawing and split charging for good amplification.