Manual

Product Number: RNK5301

Shipping and Storage

1. Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
2. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to air, each solution should be covered tightly in a timely manner after use

Components

Component	Storage	RNK5301 50Preps
Buffer CLB	RT	50 ml
Buffer RLT Plus	RT	25 ml
Buffer RW1	RT	40 ml
Buffer RW	RT	10 ml
RNase-free H2O	RT	10 ml
Genomic DNA clearance column and collection tube	RT	50
RNase free adsorption column RA and collection tube	RT	50

Description

On the basis of our company's exclusive introduction of EASYspin phenol free and chloroform based rapid RNA extraction technology, we have also independently developed the genome DNA clearance column technology, which can effectively remove gDNA residues. The obtained RNA generally does not require DNase digestion and can be used for reverse transcription PCR, fluorescence quantitative PCR and other experiments. The unique Buffer CLB and Buffer RLT Plus rapidly lyse cells and inactivate cell RNA enzymes. Centrifuge precipitation is used to remove polysaccharides, polyphenols, and secondary metabolites. Then, the mixture is lysed and ethanol is used to regulate RNA binding and adsorption onto the genomic DNA clearance column. RNA is selectively washed and filtered, and the residual DNA adsorbed on the genomic DNA clearance column cannot be washed off. The column is discarded together to remove DNA. After adjusting the binding conditions with ethanol, the filtered RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins. Finally, low salt RNase free H2O washes the pure RNA off the silica matrix membrane.

Features

1. No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
2. Simplicity, single sample operation can generally be completed within 25 minutes, making it the simplest and fastest reagent kit in the world.
3. The independently developed genomic DNA clearance column technology can effectively remove gDNA residues, and the obtained RNA generally does not require DNase digestion and can be used for experiments such as reverse transcription PCR and fluorescence quantitative PCR.
4. The world's leading adaptability is extremely extensive, and it can extract hundreds of failed samples from complex Chinese herbs such as dendrobium/salvia miltiorrhiza/snow lotus/ginseng, complex starch seeds such as rice/wheat/corn seeds, complex fruits such as grapes/blueberries/strawberries/watermelon fruits, complex stress resistant plants such as holly/pine needles/sea buckthorn/Populus euphratica, complex flowers such as roses/plums/peonies, complex polysaccharide plants such as seaweed/cactus/aloe vera, lily bulbs/rice seeds, etc. The EASYspin Plus complex Plant RNA Kit has published over 150 articles. For a detailed sample list, please refer to the company's homepage product introduction or contact us to request over 150 original published articles.
5. Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 2.1~2.2 and almost no DNA residue. It can be used for RT-PCR, Northern blot, second-generation sequencing, and various experiments.

Application

Suitable for rapid extraction of total RNA from plant tissue cells, unique genomic DNA clearance column technology can effectively remove visible gDNA residues on electrophoresis. RNA can be used for reverse transcription PCR, fluorescence quantitative PCR, etc.

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