Human Heart-Specific Serine Protease (Corin) ELISA Research Kit (Quantitative)
2026-09-14
Bovine Theileria annulata ELISA Kit
2026-10-26Bacterial Genomic DNA Fast Kit
Product Number: DNK1101
Scope of application
Suitable for genomic DNA extraction from Gram-positive and Gram-negative bacteria.
Shipping and Storage
- At low temperatures, CB may precipitate and precipitate. It can be dissolved again in a 37°C water bath for a few minutes to restore clarity and transparency. After cooling to room temperature, it can be used.
- Protease K is stored in ready to use glycerol buffer and transported at room temperature. After receipt, store at room temperature not exceeding 25℃ for at least 6 months, 4℃ for 12 months, and -20℃ for 2 years.
- To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, the lid of each solution should be promptly closed after use.
Components
| Component | Storage | DNK1101 50 Preps | DNK1102 100 Preps | DNK1103 200 Preps |
| Balance Buffer | RT | 5mL | 10mL | 20mL |
| Buffer GY | RT | 10mL | 20mL | 40mL |
| Buffer CB | RT | 11mL | 20mL | 40mL |
| Buffer PE | RT | 16mL | 32mL | 64mL |
| Add absolute ethanol as indicated on the bottle label before first use. | ||||
| Buffer WB | RT | 13mL | 25mL | 50mL |
| Add absolute ethanol as indicated on the bottle label before first use. | ||||
| Buffer EB | RT | 10mL | 10mL | 20mL |
| Proteinase K | 4°C | 1mL | 1ml ×2 | 1ml ×4 |
| Adsorption column AC | RT | 50 | 100 | 200 |
| Collection tube (2ml) | RT | 50 | 100 | 200 |
This reagent kit can be stored at room temperature for 12 months without affecting its effectiveness.
Description
The unique binding buffer and Proteinase K rapidly lyse cells. The silica-membrane spin column specifically adsorbs DNA. Toxic reagents such as phenol-chloroform and time-consuming alcohol precipitation are not required, which maximally removes proteins and other inhibitory impurities. It is suitable for efficient extraction of genomic DNA from a variety of Gram-positive and Gram-negative bacteria. The extracted DNA can be directly used for restriction digestion, PCR, Southern Blot and other experiments.
Features
- No need to use toxic phenol or other reagents, and no need for time-consuming ethanol precipitation.
- It delivers high extraction efficiency and high DNA concentration. It is compatible with various Gram-positive and Gram-negative bacteria, and can successfully extract DNA from strains that fail to be extracted or yield poor results with kits from other suppliers.
- Fast, simple, and the operation of a single sample can generally be completed within 30 minutes.
- Multiple column rinses ensure high purity, with a typical OD260/OD280 ratio of 1.7-1.9 and a length of up to 30 kb-50 kb. It can be directly used for PCR, Southern blot, and various enzyme digestion reactions.
