Porcine Cysticercosis Dye-Based Quantitative PCR Kit
2025-04-03
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2025-04-07Porcine Japanese Encephalitis Virus (JEV) Nucleic Acid Detection Kit (Fluorescent PCR Method)
Product Number: DTK573
Shipping and Storage
- -20℃±5℃, stored in the dark, transported, and subjected to repeated freeze-thaw cycles no more than 5 times, with a validity period of 12 months.
- The collected or processed samples should be stored at 2℃~8℃ for no more than 24 hours; If long-term storage is required, it should be stored at -70℃ or below, with no more than 3 freeze-thaw cycles.
Component
| Component | 50T |
| JEV reaction solution | 500μL×2 |
| Enzyme solution | 50μL |
| JEV positive quality control product | 50μL |
| Negative quality control product | 250μL |
Note: Different batches of reagents cannot be mixed.
Description
This kit uses a pair of Japanese encephalitis virus specific primers, combined with a specific fluorescent probe, to perform in vitro amplification and detection of Japanese encephalitis virus RNA using one-step fluorescent RT-PCR technology, for clinical pathogen diagnosis of suspected infectious materials.
Application
Japanese encephalitis, also known as epidemic Japanese encephalitis or simply Japanese encephalitis, is a zoonotic infectious disease caused by the Japanese encephalitis virus (JEV). JEV is mainly transmitted through mosquito bites carrying the virus, and pigs are the main breeding and spreading hosts of JEV. JEV can cause miscarriage and stillbirth in pregnant sows, orchitis in boars, encephalitis in newborn piglets, and persistent high fever in fattening pigs, causing significant economic losses to the pig industry. Japanese encephalitis B is widely present in many countries around the world, posing a serious threat to human health.
This kit is suitable for detecting Japanese encephalitis virus RNA in samples and is suitable for auxiliary diagnosis of Japanese encephalitis virus infection.
Specimen collection
Within 3 hours after the death of a deceased or euthanized animal (including miscarriage and stillbirth), brain tissue (including cerebral cortex, brainstem, midbrain, hippocampus, and pons) should be collected; To test live animals, collect no less than 5mL of cerebrospinal fluid or blood from diseased animals in sterile centrifuge tubes.
