Manual

Product Number: DTL0222

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used DGV Ⅰ/Ⅱ/Ⅲ/Ⅳ RT-PCR Master Mix and Endogenous Internal Control RT-PCR Master Mix should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. Storage time should not exceed 21 days.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
NV/RV RT-PCR Master Mix	Lyophilized powder ×1 Bottle
Positive Control	Lyophilized powder ×1 Tube
Negative Control	1mL
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.
3. Add 100μL Redissolved Diluent to the Positive Control, mix well, and use a handheld centrifuge to centrifuge briefly before use.

Description

This kit uses TaqMan probe-based real-time fluorescence PCR technology to design norovirus- and rotavirus-specific primers. Combined with specific probes, it uses fluorescence PCR technology to amplify and detect norovirus and rotavirus RNA in vitro for clinical etiological diagnosis of suspected infection.This kit sets Internal control, to monitor the presence of PCR inhibitors in the samples by detecting whether the internal control is normal, so as to avoid PCR false negative result.

Application

Norovirus (NV) is a general term for viruses of the genus Norovirus in the family Calicivirus (CV). It is a zoonotic pathogen that can cause acute gastroenteritis and severe diarrhea in humans and a variety of animals worldwide. It is also an important foodborne disease pathogen. The spread of Norovirus not only poses a serious threat to human health, but also causes huge social and economic losses.

Human rotavirus belongs to groups A, B, and C. These three groups of viruses can infect both humans and animals. Rotaviruses are primarily transmitted through fecal-oral transmission, but can also be spread through human-to-human contact, including contaminated food and water. Symptoms of infection include fever, vomiting, abdominal pain, and diarrhea.

This kit is suitable for detecting rotavirus and norovirus RNA in the feces of patients suspected of infection, and is suitable for the auxiliary diagnosis of rotavirus group A & group B & group C, and norovirus type 1 & type 2 universal infection.

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