Manual

Product Number: DTL0502

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used B19 PCR Master Mix should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. Storage time should not exceed 21 days.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
B19 PCR Master Mix	Lyophilized powder ×1 Bottle
Positive Control	Lyophilized powder ×1 Tube
Negative Control	1mL
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.
3. Add 100μL Redissolved Diluent to the Positive Control, mix well, and use a palm centrifuge to centrifuge briefly before use.

Description

This kit uses TaqMan probe real-time fluorescence PCR technology, designs a pair of Human Parvovirus B19 specific primers, combines with a specific probe, and uses fluorescence PCR technology to amplify and detect the DNA of Human Parvovirus B19 in vitro, which is used for etiological diagnosis of suspected infected materials in clinic.The kit is provided with an Internal Control, which can monitor whether there is PCR inhibitor in the sample to be tested by detecting whether the internal control is normal or not, so as to avoid false negative PCR.

Application

This kit uses TaqMan probe real-time fluorescence PCR technology, designs a pair of Human Parvovirus B19 specific primers, combines with a specific probe, and uses fluorescence PCR technology to amplify and detect the DNA of Human Parvovirus B19 in vitro, which is used for etiological diagnosis of suspected infected materials in clinic. It is suitable for detecting Human Parvovirus B19 in blood samples and other samples, and is used for auxiliary diagnosis of Human Parvovirus B19 infection.

Human parvovirus B19 is one of the common viruses. The virus particle diameter is 23nm and there is no envelope. The virus belongs to the Parvoviridae family and is a member of the genus Erythrovirus. It is the pathogen that causes infectious erythema, aplastic crisis in patients with chronic hemolytic anemia, chronic anemia in immunosuppressed patients, miscarriage during pregnancy, and stillbirth.

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