Manual

Product Number: DTL1151

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
WNV RT-PCR Master Mix	Lyophilized powder ×1 Bottle
Positive Control	100μL
Negative Control	Lyophilized powder ×1 Tube
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.
3. Add 100μL Redissolved Diluent to the Negative Control, mix well, and use a handheld centrifuge to centrifuge briefly before use.

Description

This kit uses specific primers and fluorescent probes designed for the West Nile virus genome and internal control gene 18S rRNA, and uses one-step fluorescent RT-PCR technology to perform in vitro amplification and detection of West Nile virus DNA and internal control gene 18S rRNA, and is used for clinical etiological diagnosis of suspected infected samples.

Application

West Nile virus (WNV) is a non-segmented, single-stranded, positive-sense RNA virus that belongs to the genus Flavivirus in the family Flaviviridae and can cause zoonotic diseases. The main source of infection of the virus is wild birds, and the vector is Culex mosquitoes. The virus circulates in birds at high concentrations, producing viremia and infecting large numbers of mosquitoes, which are then transmitted to birds, humans and some mammals through mosquito bites.

This kit is suitable for detecting West Nile virus RNA in venous blood samples of suspected infected animals or humans, and is suitable for auxiliary diagnosis of West Nile virus infection.

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