This report outlines the performance and purity of 100mM dNTPs (dATP, dCTP, dGTP, and dTTP) under various stress conditions, including freeze-thaw cycles, thermal exposure, and long-term storage.
1. Freeze/Thaw Cycle Test
This test evaluates the stability of dNTPs when subjected to repeated freezing and thawing, simulating frequent laboratory use.
| Item | Lot No. | Original HPLC Purity | HPLC After 50 Thaw Cycles |
|---|---|---|---|
| 100mM dATP | 120201-2 | 99.20% | 98.80% |
| 100mM dCTP | 120101-2 | 99.90% | 99.60% |
| 100mM dGTP | 110401 | 99.50% | 99.20% |
| 100mM dTTP | 110303-1 | 99.80% | 99.40% |
2. Thermal Stability Test
This test measures the degradation of dNTPs when stored at room temperature (25℃) and elevated temperature (37℃) for 10 days.
| Item | Lot No. | Original HPLC | 25℃ (10 Days) | 37℃ (10 Days) |
|---|---|---|---|---|
| 100mM dATP | 101101 | 99.60% | 99.40% | 95.00% |
| 100mM dCTP | 101101 | 99.80% | 99.90% | 96.70% |
| 100mM dGTP | 101101 | 99.70% | 99.50% | 95.00% |
| 100mM dTTP | 101101 | 99.50% | 99.40% | 95.60% |
3. Long-Term Stability Test
Data represents the purity maintained over a 5-year period under standard storage conditions.
| Item | Lot No. | Original | 1 Year | 2 Years | 3 Years | 4 Years | 5 Years |
|---|---|---|---|---|---|---|---|
| 100mM dATP | 090901-1 | 99.80% | 99.68% | 99.60% | 99.54% | 99.27% | 99.30% |
| 100mM dCTP | 090301-1 | 99.82% | 99.73% | 99.70% | 99.65% | 99.60% | 99.57% |
| 100mM dGTP | 90101 | 99.54% | 99.47% | 99.44% | 99.39% | 99.35% | 99.36% |
| 100mM dTTP | 91001 | 99.77% | 99.63% | 99.60% | 99.59% | 99.31% | 99.28% |