Manual

Product Number: DTL1411

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
PAT PCR Master Mix	Lyophilized powder ×1 Bottle
Positive Control	100μL
Negative Control	1mL
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.

Description

This kit adopts real-time fluorescent PCR technology based on the TaqMan probe method. A pair of specific primers and a specific probe targeting the PAT gene sequence are designed to amplify and detect the DNA of the PAT gene sequence in vitro by fluorescent PCR, so as to achieve the purpose of rapid detection.

Application

Phosphinthricin acetyltransferase (PAT) confers tolerance to the herbicide glufosinate-ammonium. It is widely used in the modification of rice, soybean, corn, wheat and other crops to generate a large number of glufosinate-resistant crops. This kit is suitable for the detection of the PAT gene in genetically modified plants, and is used to screen for the presence of PAT in plant samples.

---