Manual

Product Number: DTL1440

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
Cry3A PCR Master Mix	Lyophilized powder ×1 Bottle
Positive Control	100μL
Negative Control	1mL
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.

Description

This kit utilizes TaqMan probe-based real-time fluorescent PCR technology. A pair of specific primers and a corresponding specific probe are designed targeting the Cry3A gene sequence to amplify and detect Cry3A DNA in vitro by fluorescent PCR, thereby achieving rapid detection.

Application

Cry3A: an insect-resistant toxic protein that selectively kills the Colorado potato beetle. It is widely used in the screening and detection of transgenic corn, potato and other crops.

This kit is suitable for the detection of the Cry3A gene in transgenic plants and is used to screen for the presence of Cry3A in test samples.

---