Manual

Product Number: ELK00J0023

Shipping and Storage

Store at 2-8℃, away from light and moisture. Validity period: 6 months.

Component

Component	96-Well Configuration	48-Well Configuration
Microplate (96-well, pre-coated)	12 holes×8 strips	12 holes×4 strips
Standards	0.3mL×6 pipes	0.3mL×6 pipes
Sample diluent	6mL	3mL
HRP-Conjugated Detection Antibody	10mL	5mL
20× washing buffer	25mL	15mL
TMB Substrate A	6mL	3mL
TMB Substrate B	6mL	3mL
Stop solution	6mL	3mL
Plate Sealer	2 sheets	2 sheets

Note: The concentrations of standard samples (S0-S5) are 0, 15, 30, 60, 120, and 240 ng/mL, respectively.

Description

The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the sample, standard, and HRP labeled detection antibody to the pre coated micropores of hamster CHO cell host protein (CHO-HCP) antibody, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with the hamster CHO cell host protein (CHO-HCP) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.

---