Manual

Product Number: DRK0102

Shipping and Storage

1. All solutions should be clear. If the ambient temperature is low and the solution may form a precipitate, it should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity. It should be restored to room temperature before use.
2. Storing at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature.
3. To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, the lid of each solution should be promptly closed after use.

Components

Component	Storage	DRK0101 50Preps	DRK0102 100 Preps	DRK0103 200Preps
Balance Buffer	RT	5mL	10mL	20mL
Buffer DD	RT	40mL	75mL	150mL
Buffer WB	RT	13mL	25mL	50mL
		Add the specified amount of ethanol according to the bottle label instructions before the first use
Buffer EB	RT	10mL	15mL	15mL
Adsorption column EC	RT	50	100	200
Collection tube(2mL)	RT	50	100	200

This reagent kit can be stored at room temperature for 12 months without affecting its effectiveness.

Description

In the presence of highly sequenced salt, DNA fragments after dissolution of agarose gel are selectively adsorbed on the silicon matrix membrane in the centrifuge column, and then through a series of rapid rinsing centrifugation steps, the rinsing solution will remove primers, nucleotides, proteins, enzymes and other impurities, and finally the low salt, high pH elution buffer will elute pure DNA from the silicon matrix membrane.

Features

1. All the silicon matrix membranes in the centrifugal adsorption column are made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawbacks of unstable membrane quality in domestic reagent kits.
2. High quality sol solution was used, without the traditional sodium iodide and perchlorate of sol solution, which does not inhibit downstream reactions such as post harvest enzyme digestion and ligation cloning.
3. The addition of phenol red to the sol solution resulted in a yellow color, making it easier to observe the sol effect and monitor changes in pH value to achieve the best binding effect, greatly improving the yield efficiency.
4. The improved sol-gel formula greatly enhances buffering capacity and stability, allowing for pH buffering within the optimal binding range even with significant sample changes, without the need for acetic acid to adjust pH.
5. Fast, convenient, and does not require the use of toxic reagents such as phenol and chloroform, nor does it require ethanol precipitation.

---