Manual

Product Number: DTL0240

Shipping and Storage

1. Store below 30℃. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, primers, probes, dNTPs, and Mg²⁺.

Description

This kit uses TaqMan probe real-time fluorescence PCR technology to design human rotavirus group A/group B/group C specific primers, combined with specific probes, and uses fluorescence PCR technology to perform in vitro amplification and detection of human rotavirus group A/group B/group C RNA for clinical etiological diagnosis of suspected infection.

Application

Rotavirus diarrhea is a gastrointestinal infectious diarrhea caused by rotavirus. Rotavirus mainly attacks children under 5 years old from November to May of the following year and is one of the main causes of death in children in autumn and winter. Almost all children have been infected with rotavirus before the age of 5. According to statistics, there are about 111 million to 135 million cases of rotavirus diarrhea worldwide each year, resulting in the death of 650,000 infants.

This kit is suitable for detecting human rotavirus group A/group B/group C RNA in clinical samples and is suitable for auxiliary diagnosis of human rotavirus group A/group B/group C infection.

Applicable instruments

Real-time fluorescence PCR instrument with FAM,VIC,ROX,CY5 channels.

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