Manual

Product Number: DTL0217

Shipping and Storage

1. Store below 30℃. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.

Description

This kit uses real-time fluorescence PCR detection technology to achieve qualitative detection of norovirus in fecal samples by using a pair of specific primers and a specific fluorescent probe in the conserved region of the norovirus gene.This kit sets Internal control, to monitor the presence of PCR inhibitors in the samples by detecting whether the internal control is normal, so as to avoid PCR false negative result.

Application

Norovirus (NV) is a general term for viruses of the genus Norovirus in the family Calicivirus (CV). It is a zoonotic pathogen that can cause acute gastroenteritis and severe diarrhea in humans and a variety of animals worldwide. It is also an important foodborne disease pathogen. The spread of Norovirus not only poses a serious threat to human health, but also causes huge social and economic losses.

This kit is suitable for the qualitative detection of norovirus nucleic acid in fecal samples in vitro.

Applicable instruments

Real-time fluorescence PCR instrument with FAM channel and VIC channel.

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