Hantavirus Cardiopulmonary Syndrome (HCPS) PCR Kit
2026-05-14
Human P-cresyl sulfate ELISA Kit (Quantitative)
2026-05-14Human Hantavirus (HV) ELISA Kit (Qualitative)
Product Number: ELK12J0991
Shipping and Storage
- The components of the reagent kit should be stored at 2-8℃;
- Before use, please ensure that the reagent kit is stored according to the specified conditions, and only prepare the required amount of reagents for each experiment;
- Preparation of 1×Wash Solution: If crystals precipitate from the concentrated wash solution (20×), it needs to be heated at 37℃ until all crystals are dissolved before use. Dilute with distilled water at a ratio of 1:20, for example: add 10mL of concentrated washing solution to 190mL of distilled water and mix evenly. The prepared washing solution can be stored at room temperature for one week.
Component
| Component | Kit Components | 48T | 96T |
| Microplate stripplate | Wrapping board | 8×6 strips | 8×12 strips |
| Positive control | Positive control | 0.5mL | 1mL |
| Negative control | Negative control | 0.5mL | 1mL |
| Enzyme markers | HRP enzyme labeled anti human IgG | 5mL | 10mL |
| Sample diluent | PBS, BSA, etc | 5mL | 10mL |
| 20×washing buffer | 20×washing buffer | 15mL | 25mL |
| Chromogenic agent | H2O2, TMB | 6mL | 10mL |
| Stop Solution | Dilute sulfuric acid, anti precipitation agent | 3mL | 6mL |
Note: If you need to purchase universal components separately, please provide the corresponding component names.
Description
The kit employs the indirect method principle: Hantaan virus recombinant antigens are coated on the enzyme immunoassay plate. Samples are added, and IgG antibodies in the samples are captured to form "antigen-IgG antibody" immune complexes. Free impurities are washed away, followed by the addition of HRP-labeled enzyme-conjugated antibodies to create "antigen-IgG antibody-enzyme-conjugated antibody" sandwich complexes. Excess enzyme-conjugated antibodies are then washed away. TMB is added for color development, and a stop solution is subsequently added. The OD value is measured at 450nm using a microplate reader. The intensity of the color is directly proportional to the concentration of the target antibody in the sample.
Application
This kit is designed for the detection of human hantavirus IgG antibodies in serum and plasma, for research purposes only, not for diagnostic use.
Specimen collection and storage
The sample collection and storage conditions listed below are intended as general guidance, and the stability of storage and use of different types of samples has not been fully evaluated.
- Serum: Collect whole blood using a serum collection tube, let it stand at room temperature for blood cell agglutination, or centrifuge directly at 1000×g for 15 minutes to obtain serum. The operation should be gentle to avoid hemolysis. The obtained serum should be tested in a timely manner. If it cannot be tested in a timely manner, it should be divided into multiple parts and stored at a temperature of -20℃ or below for no more than 6 months. The frozen storage of serum samples should not exceed 2 freeze-thaw cycles.
- Plasma: Collect plasma using EDTA or heparin blood collection tubes. After collecting whole blood, centrifuge at 1000×g for 15 minutes to obtain plasma. The operation should be gentle to avoid hemolysis. The obtained plasma should be tested in a timely manner. If it cannot be tested in a timely manner, it should be divided into multiple parts and stored at a temperature of -20℃ or below for no more than 6 months. The frozen plasma samples should not be thawed more than twice.
- Cannot detect samples containing sodium azide, suspended fibrin or aggregates, and severe hemolysis.
- The sample should be free from microbial contamination.
