N1-methyl-pseudouridine (1-Methylpseudouridine), a methylpseudouridine, outperforms 5 mC and 5 mC/N1-methyl-pseudouridine in translation. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density.
(N1-Me-Pseudouridine-5'-Triphosphate trisodium salt solution)
|Appearance||Clear colorless solution|
|31P NMR(D2O)||To conform structure|
|1H NMR(D2O)||To conform structure|
|pH(in buffer solution)||7.0±0.1|
Please store in -20°C. Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.
1. The concentration is verified by Uv-vis spectrophotometry.
2. This lot of N1-Me-Pseudo-UTP is free of DNase and RNase contamination.
3. The purity of this preparation is determined by HPLC.