λ Phage Genome Kit
2024-12-05Soil DNA Fast Kit
2024-12-05Clinical DNA Mini Kit
Product Number: DNK2501
Shipping and Storage
- Buffer CB or Buffer IR may precipitate and precipitate at low temperatures. It can be dissolved again in a water bath at 37℃ for a few minutes to restore clarity and transparency. After cooling to room temperature, it can be used.
- Protease K is stored in a ready to use glycerol buffer and transported at room temperature. After receipt, store at room temperature not exceeding 25℃ for at least 6 months, at 4℃ for 12 months, and at -20℃ for 2 years.
- To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, each solution should be covered tightly in a timely manner after use. Carefully read Note 4.
Components
| Component | Storage | DNK2501 50 Preps |
| Buffer ML | RT | 11 ml |
| Buffer CB | RT | 15 ml |
| Buffer IR | RT | 25 ml |
| Buffer WB | RT | 13 ml |
| Poly Carrier | -20℃ | 200μl |
| Buffer EB | RT | 10 ml |
| Protease K | 4℃ | 1ml |
| Ultratrace DNA centrifuge column | RT | 50 |
Description
This reagent kit adopts a specially designed ultra trace DNA adsorption column and a unique buffer system, which is particularly suitable for isolating and purifying genomic DNA from trace samples such as blood, forensic materials, dry blood points, medical swabs, chewing gum, urine, etc. After various sources of samples are lysed and digested, DNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state (especially equipped with Poly Carrier, which can easily capture trace amounts of nucleic acids from the system). Then, impurities such as salt, cellular metabolites, proteins, etc. are removed through a series of rapid rinsing centrifugation steps. Finally, pure genomic DNA is eluted from the silica matrix membrane using low salt Buffer EB. The purified DNA is free of impurities and PCR inhibitors, making it directly suitable for PCR analysis.
Features
- The special ultra trace 5μg centrifugal column design can achieve a minimum of 6μl elution, greatly increasing the concentration of DNA.
- No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.
- Time saving, simple, and single sample Protocol can generally be completed within 20 minutes.
- Equipped with Poly Carrier for fully collecting special trace amounts of DNA.
- Multiple column washes ensure high purity, the extracted DNA has high purity, stable and reliable quality, and can be used for various routine Protocols, including PCR, enzyme digestion, sequencing, Southern hybridization, etc.
Application
Suitable for isolating and purifying genomic DNA from trace samples such as blood, forensic materials, dry blood points, and medical swabs.
