Product Number: DNK52
Shipping and Storage
Transportation conditions: room temperature; Storage conditions: Store at 2-30℃.
Components
Component | 10T | 50T |
Buffer FG1 | 35mL | 175mL |
Buffer Lysis A | 2mL | 10mL |
Buffer Lysis B | 2mL | 10mL |
Nucleic Acid Enhancer | 1mL | 5mL |
Buffer AW2 | 3mL | 15mL |
Proteinase K(20 mg/mL) | 0.14mL | 0.7mL |
RNase A(100 mg/mL) | 0.07mL | 0.35mL |
Buffer TB | 3mL | 15mL |
Nucleic acid beads | 20 pieces | 100 pieces |
Container | 10 of them | 50 of them |
Description
This kit is capable of extracting complete high molecular weight DNA from whole blood and cultured cell samples. The reagent kit innovatively uses optimized lysis buffer to specifically bind the extracted DNA A released from the sample to the surface of specially modified nucleic acid beads. The extraction time is as fast as 30 minutes (30 minutes for cell samples and about 60 minutes for blood samples), enabling rapid and efficient extraction of high molecular weight DNA. The extracted DNA length is greater than 50 kb and can reach up to Mb. The purified high molecular weight DNA has high yield and purity, and is suitable for various downstream applications such as third-generation long read sequencing, optical profiling technology, and chain read genome assembly.