Manual

Product Number: PCRD002

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Description

 PCRD, also known as Nucleic Acid Lateral Flow (NALF), is a technology that uses immunochromatography to rapidly and visually detect effectively amplified DNA. When combined with technologies such as RPA and LAMP, it enables convenient nucleic acid testing without the need for specialized laboratory equipment, significantly expanding the range of applications for nucleic acid testing. PCRD002 lateral flow Cassette Used for detecting two genes (or two loci). This test strip is suitable for use with biotin FITC/FAM、 The detection of nucleic acid amplification products with third markers is suitable for PCR amplification, isothermal amplification (RPA, LAMP, etc.).

Through optimization of the reaction system, the PCRD002 kit eliminates the need for a loading buffer. Amplified nucleic acid samples can be diluted directly with deionized water and loaded for testing. Since testing can be performed without a loading buffer, this significantly improves the convenience of product shipping and the ease of use for downstream applications.

Detection Principle

This product uses colloidal gold lateral chromatography double antibody sandwich method to rapidly detect nucleic acid amplification products. The detected nucleic acid amplification product must be labeled with biotin, FITC/FAM, or a third marker at the same time. When the double labeled amplification product flows through the test strip, it can be recognized by colloidal gold labeled antibodies and nitrocellulose membrane immobilized antibodies, forming a red band in the corresponding area.

Features

1. All-solid format—no liquids—for easy transport
2. Safe to use—eliminates the need for dye loading and UV irradiation
3. Compatible with multiple amplification formats
4. No special reagents or equipment required
5. Flexible operation—nucleic acid testing can be performed anytime, anywhere
6. Results available in as little as 10 minutes
7. Higher sensitivity than gel electrophoresis
8. Capable of testing multiple strips simultaneously
9. Stable at room temperature for up to two years

Relate

PCRD001, PCRD lateral flow strips, Single target

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