Manual

Product Number:DTK326

Shipping and Storage

-20℃ ± 5℃, avoid repeated freezing and thawing (no more than 5 cycles). Valid for 12 months.

For short-term storage, samples can be kept at -20℃. For long-term storage, use -70℃, but do not exceed 6 months. During transportation, samples should be shipped with ice packs, and repeated freezing and thawing must be avoided.

Component

Note: Different batches of reagents cannot be mixed.

Description

Enterovirus Type 71 (EV-71) belongs to the small RNA virus species and is one of the main pathogens causing hand, foot, and mouth disease (HFMD) in infants and young children. EV-71 infection clinically manifests as herpes on the hands, feet, mouth, and other areas, and can also lead to various neurological diseases such as aseptic meningitis, brainstem encephalitis, and paralysis of the spinal cord and skin[1]. EV-71 is primarily transmitted through oral routes, with a high proportion of asymptomatic carriers, making outbreaks more likely. Rapid, simple, and efficient detection methods are crucial for effective disease prevention and control[1-3]

This kit is suitable for detecting Enterovirus Type 71 RNA in fecal or diarrheal samples and serves as an auxiliary diagnostic tool for EV-71 infections.

Application

This kit is designed based on the conserved region of the Enterovirus Type 71 gene and utilizes specific probes for biological species. It employs a one-step fluorescent RT-PCR technology to amplify and detect EV-71 RNA in vitro, thereby improving the etiological diagnosis of patients in clinical settings.

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