Manual

Nucleic Acid Detection Kit (Dual Fluorescent PCR Method)

Product Number:DTK436

Shipping and Storage

1. -20℃± 5℃, stored in the dark, transported, and subjected to repeated freeze-thaw cycles no more than 5 times, with a validity period of 12 months.
2. The above specimens can be stored at -20 ℃ in the short term and -70 ℃ in the long term, but cannot exceed 6 months. The specimens should be transported in 2-8 ℃ ice packs, and repeated freezing and thawing are strictly prohibited.

Component

Note: Different batches of reagents cannot be mixed.

Description

Torque Teno Sus (TTSuVs) is a small, non enveloped, single stranded closed circular DNA virus with high genetic diversity. According to nucleotide sequence differences, TTSuV can be divided into two genotypes: TTSuV1 and TTSuV2. TTSuV1 belongs to the Iotatorquevirus genus and has two different genotypes, TTSuV1a and TTSuV1b, while TTSuV2 belongs to the Kappatorquevirus genus and has two different genotypes, TSuVk2a and TTSuVk2b. TTSuV can infect healthy or diseased pig herds. Co infection of TTSuV with circovirus type 2 (PCV2) can cause multiple system wasting syndrome (PMWS) in weaned piglets, and co infection with porcine respiratory and reproductive syndrome virus (PRRSV) can cause dermatitis nephropathy syndrome (PDNS). The pathogenic mechanism is not yet clear.

This kit is suitable for detecting porcine circovirus DNA in specimens such as lymph nodes, spleen, lungs, and serum, and is suitable for auxiliary diagnosis of porcine circovirus infection.

Application

This kit uses porcine circovirus specific primers, combined with specific fluorescent probes, to perform in vitro amplification and detection of porcine circovirus DNA using one-step fluorescent RT-PCR technology, for clinical pathogen diagnosis of suspected infectious materials.

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