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Product Number: DTK249
Shipping and Storage
Low temperature transportation, stored at -20℃, with a shelf life of 24 months.
Component
| Component | Specification | Nine hole box packaging |
| 2×Probe qPCR MasterMix | 500μL | Natural color tube |
| Fluorescent PCR specific template diluent | 1mL | Green cover |
| Ultrapure water | 1mL | Blue cover |
| Salmonella universal qPCR primer probe dry powder | 50 | Brown cover |
| Salmonella universal qPCR positive control (1 × 1E7 copy/μL) | 50μL | Yellow cover |
Note: Primer probe dry powder needs to be briefly centrifuged before use, and then 165μL of ultrapure water should be added to the centrifuge tube and thoroughly mixed before use. Unused samples need to be stored at -20℃.
Description
Salmonella spp. is a Gram negative bacterium. Salmonella infection is a zoonotic infectious diseases, which is mainly caused by eating contaminated food and is an important source of food poisoning in many countries. Typical symptoms include fever, nausea, vomiting, diarrhea, and abdominal cramps, which usually improve within 72 hours after fever. Infants, elderly people, and immunocompromised patients may experience severe and life-threatening bacteremia due to Salmonella entering the bloodstream, and a few may also develop meningitis or osteomyelitis. Therefore, rapid detection of Salmonella is of great significance for universal use.
Application
This product is a universal kit developed based on qPCR technology for the detection of Salmonella. It has the following characteristics:
- Ready to use, users only need to provide a sample DNA template.
- Primers and probes have been optimized for high analytical sensitivity, reaching up to 100 copies per reaction.
- Provide positive controls to distinguish false negative samples.
- High specificity, primers are designed based on the highly conserved region of Salmonella universal DNA and will not cross react with DNA from other organisms.
- It can be used for both qualitative and quantitative testing. When used for quantitative detection, the linear range should be at least 5 orders of magnitude.
- This product is sufficient for 50 fluorescent quantitative PCR reactions using a 20μL probe system.
- This product can only be used for scientific research.
Specimen collection
Sample DNA.
