Manual

Product Number: PSE109

Shipping and Storage

1. Storage temperature: -20±5℃ for storage
2. Validity period: 2 years.

Component

Component	PSE109
PNGase F	30μL
10×Buffer 1	1mL
10×Buffer 2	1mL
NP-40	1mL

Description

N-glycosidase F (PNGase F) is an effective amidase produced by recombinant Escherichia coli with cloned genes encoding the enzyme. It can cleave the N-sugar chain on glycoproteins, helping to generate carbohydrate free peptides and oligosaccharides with di-N-acetyl chitosan units. N-glycosidase F is the most effective method for removing almost all N-sugar chains from glycoproteins. The product can remove all complex, heterozygous, and high mannose sugar chains from antibodies and their fusion proteins, but if there are alpha 1,3-linked fucose residues in the core structure (often expressed in plant and insect cells as immunoglobulins), they cannot be cleaved. Obtaining accurate N-sugar chain distribution in the shortest possible time is crucial for effective process control. N-glycosidase F is an optimized reagent that can rapidly deglycosylate antibodies and fusion proteins within a few minutes. All N-sugar chains can be released quickly and without preference, and can be directly subjected to downstream chromatography or mass spectrometry analysis to quickly determine the glycosylation of antibodies.

Active definition

Unit enzyme activity is defined as the amount of enzyme required to remove over 95% of sugar chains from 10μg denatured RNase B within 1 hour in a 10μL reaction system at 37℃.

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