Manual

Product Number: DTK325

Shipping and Storage

1. -20℃±5℃, stored in the dark, transported, and subjected to repeated freeze-thaw cycles no more than 5 times, with a validity period of 12 months.
2. The collected or processed samples should be stored at 2℃~8℃ for no more than 24 hours; If long-term storage is required, it should be stored at -70℃ or below, with no more than 3 freeze-thaw cycles.

Component

Note: Different batches of reagents cannot be mixed.

Description

This kit uses a pair of hepatitis A virus specific primers, combined with a specific fluorescent probe, to perform in vitro amplification and detection of hepatitis A virus RNA using one-step fluorescent RT-PCR technology, for clinical pathogen diagnosis of suspected infectious materials.

Application

Hepatitis A is an intestinal infectious disease caused by the hepatitis A virus (HAV). Distributed worldwide, China is a high-risk area with the highest incidence of various types of hepatitis. The traditional detection of hepatitis A virus is through cell culture. Due to the long culture cycle and low proliferation ability of HAV cells, they generally do not cause cell damage and are difficult to meet the detection requirements. PCR technology has improved sensitivity and specificity, and greatly shortened detection time, bringing convenience to hepatitis A detection [1-3].

This kit is suitable for detecting hepatitis A virus RNA in blood and other samples of suspected infected individuals, and is suitable for auxiliary diagnosis of hepatitis A virus infection.

Sample requirements

2mL of venous blood from suspected infected individuals is transferred to EDTA-2Na anticoagulant tubes.

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