Manual

Product Number: DTL0306

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used DGV Ⅰ/Ⅱ/Ⅲ/Ⅳ RT-PCR Master Mix and Endogenous Internal Control RT-PCR Master Mix should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. Storage time should not exceed 21 days.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
DGV Ⅰ/Ⅱ/Ⅲ/Ⅳ RT-PCR Master Mix	Lyophilized powder ×1 Bottle
Endogenous Internal Control RT-PCR Master Mix	Lyophilized powder ×1 Bottle
Positive Control	Lyophilized powder ×1 Tube
Negative Control	1mL
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.
3. Add 100μL Redissolved Diluent to the Positive Control, mix well, and use a handheld centrifuge to centrifuge briefly before use.

Description

This kit uses the multiplex fluorescent PCR detection technology to design respectively specific primers and probes for Dengue virus I, Dengue virus II, Dengue virus III, and Dengue virus IV, to realize the qualitative detection and genotyping identification of Dengue virus I/II/III/IV in serum samples.

Application

This kit is suitable for in vitro qualitative detection of dengue virus type I/II/III/IV nucleic acid in human serum samples. The test results can be used for the typing and identification of dengue virus type I/II/III/IV. The test results are not the only indicators for the evaluation of the patient's condition, and the condition must be comprehensively analyzed in combination with the patient's clinical manifestations and other laboratory tests.

Dengue is an acute febrile illness caused by the dengue virus and is characterized by fever, arthralgia, muscle pain, rash, swollen lymph nodes, and leukopenia. Dengue virus belongs to the Flavivirus genus in the Flaviviridae family. Virus particles are dumbbell-shaped, rod-shaped or spherical, and the genome is single-stranded positive-stranded RNA. In clinical or laboratory, virus cell culture method or neutralization test is used as the main detection method.

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