Manual

Product Number: DTL0401

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
HBV PCR Master Mix	Lyophilized powder ×1 Bottle
Positive Control	100μL
Negative Control	1mL
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.

Description

This kit utilizes real-time fluorescent PCR detection technology, and uses specific primers and probes for HBV to achieve qualitative detection of HBV DNA in serum samples. The kit is provided with an Internal Control（IC）, which can monitor whether there is PCR inhibitor in the sample to be tested by detecting whether the internal control is normal or not, so as to avoid false negative PCR.

Application

This kit is suitable for the qualitative detection of hepatitis B virus nucleic acid (HBV DNA) in human serum samples in vitro, and the test results are used for the auxiliary diagnosis of hepatitis B. The test results are not the only indicators for the evaluation of the patient's condition, and the condition must be comprehensively analyzed in combination with the patient's clinical manifestations and other laboratory tests.

Hepatitis B is a worldwide epidemic and is a group of infectious diseases characterized by liver damage caused by Hepatitis B Virus (HBV).It has the characteristics of strong infectivity, complex transmission route, wide prevalence and high incidence. The main clinical manifestations are fatigue, loss of appetite, nausea, vomiting, hepatomegaly and abnormal liver function. After a person is infected with HBV, those who have not cleared the virus for 6 months are called chronic HBV infection. Without appropriate intervention, some infected persons may develop liver failure, cirrhosis and primary hepatocellular carcinoma.

---