Manual

Product Number: DTL0727

Shipping and Storage

1. Store below 30℃. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, primers, probes, dNTPs, and Mg²⁺.

Description

This kit uses specific primers for Neisseria meningitidis X, W, and Y types, respectively combined with a specific fluorescent probe, and uses one-step fluorescent RT-PCR technology to perform in vitro amplification and detection of DNA of Neisseria meningitidis X, W, and Y types, and is used for clinical etiological diagnosis of suspected infectious materials.

Application

Neisseria meningitidis is a Gram-negative, encapsulated bacterium that can cause sepsis, meningitis, and death. Based on a chemically and antigenically distinct polysaccharide capsule, Neisseria meningitidis can be classified into at least 12 serogroups (including serogroups A, B, C, 29E, H, I, K, L, W-135, X, Y, and Z). Strains with five of these serogroups (A, B, C, Y, and W135) cause the majority of disease.

This kit is suitable for detecting DNA of Neisseria meningitidis type X, type W, and type Y in samples of cerebrospinal fluid, blood, and throat swabs, and is suitable for auxiliary diagnosis and epidemiological investigation of Neisseria meningitidis type X, type W, and type Y infection.

Applicable instruments

Real-time fluorescence PCR instrument with FAM,VIC,ROX,CY5 channels.

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