Even Green (Eva Green) Nucleic Acid Gel Stain (20x)
2026-07-03
Diagnostic Kit for Neisseria Meningitidis A/B/C DNA
2026-07-034th Generation safe stain
Product Number: ST08
Description
Safe Stain Nucleic Acid Gel Stain is a kind of new generation of fluorescent nucleic acid gel stain designed to replace the highly toxic ethidium bromide(EtBr). The Ames test confirmed that are nonmutagenic at concentratons well above their working concentrations used for gel staining. Nucleic Acid Gel Stain is highly sensitive than EtBr either as precast gel stains or post gel stains.
Safe Stain and EB have virtually the same spectra, so you can directly replace EB with Safe Stain without changing your existing imaging system. Safe Stain cannot be sufficiently excited with a 488nm argon laser or similar visible light. Safe Stain can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. Precast polyacrylamide gel staining with Safe Stain is not recommended because of relatively high background fluorescence.
Safe Stain Nucleic Acid Gel Stain 10,000x in DMSO is a concentrated solution that can be diluted 10,000 times for use in precast gel staining for ~3,300 times for use in post gel staining according to the procedures described below. One vial (0.5ml) of 10,000x solution can be used to prepare at 100 precast minigels or post-stain at least 100 minigels.
Gel staining with Safe Stain is compatible with downstream appications such as gel extraction and cloning. Safe Stain is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.
Features
- Safety: Nonmutagenic and noncytotoxic.
- Easy disposal: Safe to dispose in the drain.
- Compatibility: Spectrally compatible with existing instruments.
- Sensitivity: Higher signal but lower background.
- Stability: can be stored at RT and microwavable.
