SafeGreen Nucleic Acid Gel Stain is a kind of new generation of fluorescent nucleic acid gel stain designed to replace the highly toxic ethidium bromide (EtBr). The Ames test confirmed that SafeGreen are nonmutagenic at concentrations well above their working concentrations used for gel staining. SafeGreen Nucleic Acid Gel Stain are highly sensitive than EtBr either as precast gel stains or post gel stains.
SafeGreen has UV absorption between 250nm and 300nm and a strong absorption peak centered around 500nm and is compatible with either a 254nmUV transilluminator or a gel reader equipped with visible light excitation(such as a 488nm laserbased gel scanner or a Dark Reader).
SafeGreen Nucleic Acid Gel Stain 10,000X in DMSO is a concentrated SafeGreen solution that can be diluted 10,000 times for use in precast gel staining for ~3,300 times for use in post gel staining according to the procedures described below. One vial(0.5ml) of 10,000X solution can be used to prepare at 100 precast minigels or post-stain at least 100 minigels.
Gel staining with SafeGreen is compatible with downstream applications such as gel extraction and cloning. SafeGreen is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.