SafeRed Nucleic Acid Gel Stain is the third generation nonmutagenic fluorescent nucleic acid gel stain to replace the highly toxic traditional ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. The third part’s Ames test shows that SafeRed and SafeGreen are nonmutagenic for gel staining. SafeGreen and SafeRed Nucleic Acid Gel Stain are highly sensitive than EtBr either as precast gel stains or post gel stains.
We can directly replace EB with SafeRed without changing the existing imaging system since SafeRed and EB have the similar spectra. SafeRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. Gel staining with SafeRed is compatible with downstream applications such as gel extraction and cloning. SafeRed is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.