Product Number: BFE-48T
Shipping and Storage
Component
Component | 48T |
User manual | 1 |
Closure plate membrane | 2 |
Sealed bags | 1 |
Microelisa stripplate | 1 |
Standard | 0.3mL×6 bottle |
HRP-Conjugate reagent | 5mL×1 bottle |
Sample diluent | 3mL×1 bottle |
Chromogen Solution A | 3mL×1 bottle |
Chromogen Solution B | 3mL×1 bottle |
Stop Solution | 3mL×1 bottle |
20×Wash solution | 15mL×1 bottle |
Note: Standard concentration was followed by: 100、50、25、12.5、6.25、0ug/mL.
Description
The kit assay Bovine FN level in the sample, use Purified Bovine FN antibody to coat microtiter plate wells, make solid-phase antibody, then add FN to the wells, Combined antibody which With HRP labeled, become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of FN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Application
This kit allows for the determination of FN concentrations in Bovine serum, plasma, tissue homogenates and other biological fluids.