Chicken infectious bronchitis virus antibody (IB Ab) ELISA quantitative detection kit
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Product Number: IB01
Shipping and Storage
- 2-8℃, stored away from light and moisture
- Validity period: 6 months
Component
| Component | 96T | 48T |
| Microplate (96-well, pre-coated) | 12 holes×8 strips | 12 holes×4 strips |
| Negative control | 0.5mL | 0.5mL |
| Positive control | 0.5mL | 0.5mL |
| HRP-Conjugated Detection Antibody | 10mL | 5mL |
| 20× washing buffer | 25mL | 15mL |
| Sample diluent | 6mL | 3mL |
| TMB Substrate A | 6mL | 3mL |
| TMB Substrate B | 6mL | 3mL |
| Stop solution | 6mL | 3mL |
| Plate Sealer | 2 sheets | 2 sheets |
Description
The reagent kit adopts indirect enzyme-linked immunosorbent assay (ELISA). Add the specimen and HRP labeled detection antibody to the pre coated micropores of infectious bronchitis virus antigen, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and compare it with the CUTOFF value to determine the presence or absence of chicken infectious bronchitis virus antibodies (IBD Ab) in the specimen.
