Manual

Kit(Qualitative)

Product Number: ELK10746

Shipping and Storage

1. Reagent kit storage: 2-8℃, stored away from light and moisture.
2. Validity period: 6 months.

Component

Component	96T
20× concentrated wash buffer	20mL×1
Sample diluent	6mL×1
Detect antibody HRP	10mL×1
Microplate (Coated)	12 wells×8 strips
Sample diluent	6mL×1
Chromogen substrate A	6mL×1
Chromogen substrate B	6mL×1
Stop solution	6mL×1
Positive control	0.5mL×1
Negative control	0.5mL×1

Description

The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the specimen and HRP labeled detection antibody to the pre coated micropores with human immunodeficiency virus antigen, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. Measure the absorbance (OD value) at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader and compare it with the CUTOFF value to determine the presence or absence of human immunodeficiency virus (HIV) in the specimen.

Application

This kit is used to determine the expression of hepatitis B surface antigen (HBsAg) in human serum and plasma samples.

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