Human FABP4 ELISA Kit
2025-12-11Human Betatrophin ELISA Kit
2025-12-11Product Number: ELK024
Shipping and Storage
- Reagent kit storage: 2-8℃.
- Validity period: 6 months.
Component
| Component | 96T |
| 30× concentrated wash buffer | 20mL×1 |
| Enzyme conjugate | 6mL×1 |
| Microplate (Coated) | 12 wells×8 strips |
| Sample diluent | 6mL×1 |
| Chromogen substrate A | 6mL×1 |
| Chromogen substrate B | 6mL×1 |
| Stop solution | 6mL×1 |
| Standard (240ng/L) | 0.5mL×1 |
| Standard diluent | 1.5mL×1 |
Description
This kit uses the double antibody sandwich method to determine the level of human cardiotrophin-1 (CT-1) in the specimen. Coat a microplate with purified human cardiotrophin-1 (CT-1) antibody to prepare a solid-phase antibody. Add cardiotrophin-1 (CT-1) sequentially to the micropores coated with the antibody, and then bind with HRP labeled cardiotrophin-1 (CT-1) antibody to form an antibody antigen enzyme labeled antibody complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with cardiotrophin-1 (CT-1) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of human cardiotrophin-1 (CT-1) in the sample using a standard curve.
Application
This kit is used to determine the content of cardiotrophin-1 (CT-1) in human serum, plasma, and related liquid samples.
Testing Scope
5ng/L – 120ng/L
