Human Matrix Metalloproteinase-2 (MMP-2) ELISA Assay Kit
2025-11-05
Cell Counting Kit
2025-11-05Product Number: ELK009
Shipping and Storage
- 2-8℃, stored away from light and moisture
- Validity period: 6 months
Component
| Component | 96-Well Configuration | 48-Well Configuration |
| Microplate (96-well, pre-coated) | 12 holes×8 strips | 12 holes×4 strips |
| Standards | 0.3mL×6 pipes | 0.3mL×6 pipes |
| Sample diluent | 6mL | 3mL |
| HRP-Conjugated Detection Antibody | 10mL | 5mL |
| 20× washing buffer | 25mL | 15mL |
| TMB Substrate A | 6mL | 3mL |
| TMB Substrate B | 6mL | 3mL |
| Stop solution | 6mL | 3mL |
| Plate Sealer | 2 sheets | 2 sheets |
Note: The concentrations of standard samples (S0-S5) are 0, 421.875, 843.75, 1687.5, 3375, and 6750pg/mL, respectively.
Description
The kit employs the double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Pre-coated microplates with matrix metalloproteinase 9 (MMP-9) antibodies are sequentially treated with samples, standards, and HRP-labeled detection antibodies, followed by incubation and thorough washing. The substrate TMB is used for color development, where TMB is converted to a blue color under peroxidase catalysis and further transformed into a final yellow color under acidic conditions. The intensity of the color is directly proportional to the concentration of matrix metalloproteinase 9 (MMP-9) in the sample. The absorbance (OD value) is measured at a wavelength of 450 nm using a microplate reader, and the sample concentration is calculated accordingly.
