Enterovirus 71/Coxsackievirus A16 (EV71CA16) Nucleic Acid
2025-05-28
MB400H-Magnetic Bead
2025-06-03Product Number: OMPD01
Shipping and Storage
- 2-8℃, stored away from light and moisture.
- Validity period: 6 months.
Component
| Component | 96T | 48T |
| Microplate (96-well, pre-coated) | 12 holes×8 strips | 12 holes×4 strips |
| Standard Sample | 0.3mL×6 tubes | 0.3mL×6 tubes |
| Sample diluent | 6mL | 3mL |
| HRP-Conjugated Detection Antibody | 10mL | 5mL |
| 20× washing buffer | 25mL | 15mL |
| TMB Substrate A | 6mL | 3mL |
| TMB Substrate B | 6mL | 3mL |
| Stop solution | 6mL | 3mL |
| Plate Sealer | 2 sheets | 2 sheets |
Note: The concentrations of standard samples (S0-S5) are 0, 0.75, 1.5, 3, 6, and 12ng/mL, respectively.
Description
The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the sample, standard, and HRP labeled detection antibody in sequence to the pre coated micropores with OMPD protein (OmpD) antibody, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with the OMPD protein (OmpD) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.
