Manual

Product Number: ELK015001

Shipping and Storage

1. 2-8℃, stored away from light and moisture
2. Validity period: 6 months

Component

Component	96T	48T
Microplate (96-well, pre-coated)	12 holes×8 strips	12 holes×4 strips
Negative control	0.5mL	0.5mL
Positive control	0.5mL	0.5mL
Detect antibody HRP	10mL	5mL
20× washing buffer	25mL	15mL
Sample diluent	6mL	3mL
Substrate A	6mL	3mL
Substrate B	6mL	3mL
Stop solution	6mL	3mL
Plate Sealer	2 sheets	2 sheets

Description

The reagent kit adopts indirect enzyme-linked immunosorbent assay (ELISA). Add the specimen and HRP labeled detection antibody to the pre coated micro well with Treponema pallidum antigen, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. Measure the absorbance (OD value) at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader and compare it with the CUTOFF value to determine the presence or absence of Treponema pallidum antibody (TP Ab) in the specimen.

Specimen collection and storage

1. Serum: Use test tubes without pyrogen and endotoxin, avoid any cell irritation during the operation, collect blood, centrifuge at 3000 rpm for 10 minutes, and quickly and carefully separate serum and red blood cells.
2. Plasma: anticoagulant with EDTA, citrate or heparin. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant.
3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers.
4. Tissue homogenate: Crush the tissue by adding an appropriate amount of physiological saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant.
5. Storage: If the sample is not tested in a timely manner after collection, please pack it according to a single dose and freeze it at -20℃ to avoid repeated freezing and thawing. Thaw it at room temperature and ensure that the sample is evenly filled and thawed.

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