BigDye3.1 sequecing kit
2025-12-03Product Number: ELK005
Shipping and Storage
- Reagent kit storage: 2-8℃.
- Validity period: 6 months.
Component
| Component | 96T |
| 30× Concentrated Wash Buffer | 20mL×1 |
| Enzyme Conjugate | 6mL×1 |
| Microplate (Coated) | 12 wells×8 strips |
| Sample Diluent | 6mL×1 |
| Chromogen Substrate A | 6mL×1 |
| Chromogen Substrate B | 6mL×1 |
| Stop solution | 6mL×1 |
| Positive control | 0.5mL×1 |
| Negative control | 0.5mL×1 |
Description
This kit uses the double antibody sandwich method to determine the expression of Escherichia coli K99 (E.coli K99) in specimens. Using purified antibody coated microplates, solid-phase antibodies can be prepared that can bind to Escherichia coli K99 in the sample. After washing to remove unbound antigens and other components, they can then bind to HRP labeled antibodies to form antibody antigen enzyme-linked antibody complexes. After thorough washing, TMB substrate is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and compare it with the CUTOFF value to determine the presence or absence of Escherichia coli K99 in the specimen.
Application
This kit is used to determine the expression of Escherichia coli K99 (E.coli K99) in microbial samples.
Specimen requirements
- Extract the specimen as soon as possible after collection, according to relevant literature, and conduct experiments as soon as possible after extraction. If the experiment cannot be conducted immediately, the specimen can be stored at -20℃, but repeated freezing and thawing should be avoided
- Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP).
