DPBS, without Calcium and Magnesium
2025-12-10
Sheep Escherichia coli K99 IgG antibody (E.coli K99 IgG) ELISA Kit
2025-12-11Product Number: ELK006
Shipping and Storage
- Reagent kit storage: 2-8℃.
- Validity period: 6 months.
Component
| Component | 96T |
| 30× Concentrated Wash Buffer | 20mL×1 |
| Enzyme Conjugate | 6mL×1 |
| Microplate (Coated) | 12 wells×8 strips |
| Sample Diluent | 6mL×1 |
| Chromogen Substrate A | 6mL×1 |
| Chromogen Substrate B | 6mL×1 |
| Stop solution | 6mL×1 |
| Positive control | 0.5mL×1 |
| Negative control | 0.5mL×1 |
Description
This kit uses the double antigen sandwich method to determine the level of mouse Escherichia coli K99 antibody IgG (E.coli K99 IgG) in the specimen. Coat the microporous plate with purified antigen to prepare solid-phase antigen. Add E. coli K99 IgG antibody to the coated micropores in sequence, and then bind with HRP labeled antigen to form an antigen antibody enzyme labeled antigen complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with the presence of E. coli K99 IgG antibody in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of mouse Escherichia coli K99 antibody IgG (E.coli K99 IgG) in the sample using a standard curve.
Application
This kit is designed to measure the content of E. coli K99 IgG antibodies in mouse serum, plasma, and related liquid samples.
Testing Scope
1pg/mL-50pg/mL
