Ribonuclease A (RNase A) is a genetically engineered endonuclease which is originally from bovine genome. The product is produced and purified from yeast cells without contamination of endotoxin.
RNase A is a very stable endoribonuclease and a single chain polypeptide containing four disulfide bridges. Solutions of RNase A have been reported to withstand temperatures up to 100°C. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts.
A major application for RNase A is the removal of RNA from preparations of biological products, such as plasmid DNA or protein samples. It is also used in RNA sequence analysis and protection assays.
Transportation and Storage
Storage temperature: -20°C.
Storage buffer: 10 mM Tris-HCl (pH 8.0), 50% glycerol.
Dilution buffer: 10 mM Tris-HCl (pH 8.0).
RNase A is provided as a solution of 10 mg/mL.
RNase A can be used at a final concentration of 10-20 μg/mL.
|RA02||Ribonuclease A Recombinant||Recombinant Ribonuclease A solution. Animal free, DNase free, host DNA free. 10mg/ml, Activity>=350KU/ml. DNA extraction or RNA sequencing|