Proteinase K solution
2025-01-22Product Number: RA02
Description
Our Ribonuclease A Solution is a non-specific ribonuclease derived from bovine pancreas that has been modified through protein engineering technology. It is obtained through yeast expression and purification. It does not contain bacterial endotoxins expressed by prokaryotes. Functionally, RNase A is a ribonuclease that can hydrolyze the phosphodiester bond between the 5 '- ribose on a nucleoside and the phosphate group on the adjacent pyrimidine nucleoside 3' - ribose. The resulting 2 ', 3' - cyclic phosphate can be hydrolyzed into the corresponding 3 '- nucleoside phosphate. Therefore, single stranded RNA can be specifically degraded at the positions of C and U nucleotide residues. RNase A is very stable. RNase A exhibits high efficacy when acting on single stranded RNA.
This product is widely used to remove RNA contamination from DNA or protein samples.
This product is free from contamination by endonucleases and exonucleases, as well as protease contamination.
| Level | Molecular biological grade, sterile liquid |
| Product Number | RA02 |
| CAS Number | 9001-99-4 |
| E.C. | 3.1.27.5 |
| Sorce | Recombinant yeast |
| Molecular Weight | 14.3 kD |
| Dilution Buffer | 10mM Tris-HCl(pH 8.0),20mM MgCl2 |
| Storage Buffer | 10mM Tris-HCl(pH 8.0),20 mM MgCl2,50% glycerol |
| Storage | Can be transported with blue ice or at room temperature, and stored below -20℃ after receipt. |
| Shelf life | After opening the packaging and using it, if it is left in an environment of 2-8℃ for more than a week, it is recommended to filter and sterilize it to prevent microbial contamination. |
Quality Index
| Appearance | Light yellow transparent solution |
| Electrophoretic Purity | ≥90%(SDS-PAGE) |
| Activity | ≥350kunitz Units/ml |
| Specific activity | ≥1000kU/mg protein |
| DNA and RNA | Invisible |
| DNase | Invisible |
| Protease K | ＜0.01U/mg |
| Endomycin | Not detected |
| Sterility test | Not detected |
| pH | 7.6 (working range pH 6-10) |
| Optimum temperature | 60℃ (working range 15-70℃) |
| Protocol | The storage concentration is 10mg/ml. The recommended working concentration is 10-20μg/ml. The working solution should be diluted with diluent or ensure that there is 20mM Mg2+in the working substrate. |
Note
For your safety and health, please wear lab clothes and protective gloves when using.
