Ribonuclease A (RNase A) is a genetically engineered endonuclease which is originally from bovine genome. The product is produced and purified from yeast cells without contamination of endotoxin.
RNase A is a very stable endoribonuclease and a single chain polypeptide containing four disulfide bridges. Solutions of RNase A have been reported to withstand temperatures up to 100°C. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts.
A major application for RNase A is the removal of RNA from preparations of biological products, such as plasmid DNA or protein samples. It is also used in RNA sequence analysis and protection assays.
Transportation and Storage:
Storage temperature: -20°C.
Storage buffer: 10 mM Tris-HCl (pH 8.0), 50% glycerol.
Dilution buffer: 10 mM Tris-HCl (pH 8.0).
RNase A is provided as a solution of 10 mg/mL.
RNase A can be used at a final concentration of 10-20 μg/mL.