CCK-8 Cell Proliferation and Cytotoxicity Detection Kit
2025-12-11
PBS buffer
2025-12-11Product Number: S080006
Shipping and Storage
Dry ice transportation; stored at -20°C, shelf life of 12 months.
Component
| Component | S080006 |
| 50× Cocktail | 250μL |
| 0.5M EDTA | 125μL |
Description
This product, 50× Cocktail, is a mixed-type protease inhibitor containing a variety of broad-spectrum protease inhibitors, including AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, and Pepstatin A, with DMSO as the solvent. The product also includes additional EDTA for selective use. The various components in this product can effectively inhibit protease activity in samples from animals, plants, yeast, and bacteria. Among them, AEBSF is a water-soluble irreversible serine protease inhibitor with inhibition constants similar to those of PMSF and DFP. It effectively inhibits proteases such as trypsin, chymotrypsin, plasmin, kallikrein, and thrombin, serving as a substitute for PMSF and DFP with lower toxicity, better water solubility, and improved stability in aqueous solutions. Aprotinin is a competitive reversible serine protease inhibitor. Bestatin is a reversible aminopeptidase inhibitor. E-64 is an irreversible cysteine protease inhibitor. Leupeptin is a reversible serine and cysteine protease inhibitor. Pepstatin A exhibits effective inhibition against aspartic proteases such as cathepsin D and E. EDTA can inhibit metalloproteases.
This product is suitable for protein extraction from various biological samples (e.g., animal tissues/cells, plant tissues, bacteria, yeast, etc.) and is compatible with subsequent protein quantification assays, immunoblot experiments (e.g., WB, Co-IP, pull-down), and more. When diluted 50-fold, 250μL of mixed protease inhibitors can prepare 12.5mL of lysis buffer, and 125μL of EDTA can be used to prepare 12.5mL of lysis buffer.
