Manual

Product Number: DNK49

Shipping and Storage

Transport and store at room temperature, with a shelf life of one year.

Components

Description

This product is developed based on our company's blood DNA extraction product and is specifically used as a reagent for extracting mitochondrial DNA (mtDNA) from fresh or frozen human anticoagulant whole blood. The classification of cells in human blood is as follows: the ratio of red blood cells to white blood cells in human blood is 1000-6000:1, but there is no DNA in red blood cells, so the mitochondrial DNA mainly comes from white blood cells. White blood cells are divided into two types: polymorphonuclear leukocytes (PMNs) and peripheral blood mononuclear cells (PBMCs). The former includes neutrophils, which account for 50-70% of the total white blood cells, eosinophils, which account for 0.5-5%, and basophils, which account for 0-1%. The latter includes lymphocytes (T cells, B cells, and NK cells) that account for 20-40% of the total white blood cell count, monocytes (1-8%), and dendritic cells (0.3-0.9%).

Features

1. High DNA yield. If the sample is fresh blood, the highest mitochondrial DNA yield can reach 1μg/mL fresh blood. If the sample is frozen blood, the difference in DNA yield is significant.
2. The DNA is pure, with OD260/OD280 between 1.8-2.0.
3. It can be directly used for PCR, enzyme digestion, hybridization and other experiments.
4. Applicable to mammalian blood, not applicable to blood of other animals.
5. This product is sufficient for 50 uses and can handle 3mL of anticoagulant blood each time.
6. Each 1E7 white blood cell can be purified to 40-80ng mtDNA.
7. This product can only be used for scientific research.

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