Starch Casein Agar(SCA)
2025-10-13Product Number: DNK55
Shipping and Storage
- Cracking solution PF may precipitate and precipitate at low temperatures in winter. It can be dissolved again in a 55°C water bath for a few minutes to restore clarity and transparency. After that, it can be gently and thoroughly mixed and cooled to room temperature before use.
- To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, the lid of each solution should be promptly closed after use.
Components
| Component | Storage | DNK5501 50 Preps | DNK5502 100 Preps | DNK5503 200 Preps |
| RNase A | 4°C | 250μL | 500μL | 1mL |
| Buffer PF | RT | 30mL | 60mL | 120mL |
| Buffer PQ | RT | 18mL | 35mL | 70mL |
| Add the specified amount of ethanol according to the bottle label instructions before the first use | ||||
| Buffer PE | RT | 16mL | 32mL | 64mL |
| Add the specified amount of ethanol according to the bottle label instructions before the first use | ||||
| Buffer WB | RT | 13mL | 25mL | 50mL |
| Add the specified amount of ethanol according to the bottle label instructions before the first use | ||||
| Buffer EB | RT | 10mL | 10mL | 20mL |
| Adsorption column AC | RT | 50 | 100 | 200 |
| Collection tube | RT | 50 | 100 | 200 |
Note: This reagent kit can be stored at room temperature for 12 months without affecting its effectiveness.
Description
This product uses our company's unique complex plant/fungal sample DNA extraction solution (with the addition of various polysaccharides, polyphenols, and starch removal components specific to plant/fungal characteristics) to rapidly lyse cells and inactivate intracellular nucleases. After chloroform extraction, polysaccharides, polyphenols, and protein impurities are removed by centrifugation. Then, DNA is selectively adsorbed onto the silica matrix membrane in a highly ionized salt state. After multiple washes, polysaccharides, polyphenols, cellular metabolites, proteins, and other impurities are further removed. Finally, a low salt elution buffer is used to elute pure DNA from the silica matrix membrane.
Features
- All the silicon matrix membranes in the centrifugal adsorption column are made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawbacks of unstable membrane quality in domestic reagent kits.
- Widely applicable, it can extract the vast majority of samples with high quality, including the most complex polysaccharide polyphenols and starch seed samples.
- Easy to operate, a single sample operation can generally be completed in 30 minutes.
- Several methods of removing polysaccharides and polyphenols, as well as multiple column rinses, ensure high purity. The typical ratio of OD260/OD280 is 1.7-1.9, and the length can reach 20-50kb. It can be directly used for PCR, Southern blot, and various enzyme digestion reactions.
