Manual

Product Number: DNK53

Shipping and Storage

Magbeads ZN 2-8℃, other components at room temperature (10-30℃).

Component

Component	DNK53-48T
Buffer CTL	2×72mL
Buffer CTW	60mL
Buffer GCW2（concentrate）	2×10mL
RNase-Free Water	10mL
Proteinase K	5mL
Magbeads ZN	2×1mL

Description

This kit is suitable for the purification and recovery of free circulating/cell-free DNA from cell-free body fluids such as plasma, serum, and urine (1-5 mL). The kit combines lysis at room temperature with nucleic acid adsorption into a single step, eliminating the need for ice baths or pauses when adding binding buffer and magnetic beads, significantly simplifying the workflow. In the presence of high salt, free DNA binds to the surface of silica-coated magnetic beads. After washing, free DNA is eluted in enzyme-free water. The yield of free DNA is highly dependent on sample type, storage conditions, duration, and inter-individual variations. The kit features high extraction efficiency, excellent reproducibility, simple and efficient operation, and rapid processing. The purified free DNA is stable and reliable in quality, suitable for downstream routine experiments such as quantitative PCR and second-generation sequencing library construction.

---